(Salmonella Gp B/D ) SALMONELLA GROUP B AND D ANTIBODY Test Kit
The Salmonella Gp B/D ELISA kit will measure the amount of antibody to Se or St and other invasive group B and D salmonella species in the serum of chickens. Microtitre plates have been pre-coated with inactivated Se and St antigen. Chicken serum samples are diluted and added to the microtitre wells where any anti-Se or anti-St antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other serum proteins are then washed away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any chicken anti-Se or anti-St antibodies originally bound to the antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if anti-Se or anti-St antibody is present and the intensity is directly related to the amount of anti-Se or anti-St present in the sample.