(ORT) ORNITHOBACTERIUM RHINOTRACHEALE ANTIBODY Test Kit
Gamintojo informacija
The ORT ELISA kit will measure the amount of antibody to ORT in the serum of chickens and Turkeys. Microtitre plates have been pre-coated with inactivated ORT antigen. Serum samples are diluted and added to the microtitre wells where any anti- ORT antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other serum proteins are then washed away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any chicken anti- ORT antibodies originally bound to the antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if anti- ORT antibody is present and the intensity is directly related to the amount of anti- ORT present in the sample.