(Hps) HAEMOPHILUS PARASUIS ANTIBODY Test Kit
The Haemophilus parasuis (Hps OppA) antibody ELISA kit will measure the amount of antibody to OppA in the serum of pigs. Microtitre plates have been pre-coated with inactivated OppA. Samples taken from pigs are diluted and added to the microtitre wells where any anti-OppA antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other sample proteins are then washed away. Anti-pig antibody labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any Swine anti-OppA originally bound to antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if any anti-OppA antibody is present and the intensity is directly related to the amount of antibody present in the sample.