(CSFV) CLASSICAL SWINE FEVER ANTIBODY Test Kit
The Classical Swine Fever Virus antibody ELISA kit will measure the amount of antibody to CSFV E2 in the serum of pigs. Microtitre plates have been pre-coated with recombinant CSFV E2 protein. Samples taken from pigs are diluted and added to the microtitre wells where any anti- CSFV E2 antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other sample proteins are then washed away. Anti-pig antibody labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any Swine anti- CSFV E2 originally bound to antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if any anti- CSFV E2 antibody is present and the intensity is directly related to the amount of antibody present in the sample.